兔抗DLAT多克隆抗體

Background:

This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.

Applications:

ELISA, WB, IHC

Name of antibody:

DLAT

Immunogen:

Fusion protein of human DLAT

Full name:

dihydrolipoamide S-acetyltransferase

Synonyms：

DLTA; PDCE2; PDC-E2

SwissProt:

P10515

ELISA Recommended dilution:

2000-5000

IHC positive control:

Human breast cancer and Human prostate cancer

IHC Recommend dilution:

30-150

WB Predicted band size:

69 kDa

WB Positive control:

A431, hepg2 and lncap cell

WB Recommended dilution:

500-2000