小鼠抗MRPL11單克隆抗體

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 5q and 12q. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC

Name of antibody:

MRPL11

Immunogen:

Fusion protein of human MRPL11

Full name:

mitochondrial ribosomal protein L11 (MRPL11), transcript variant 1

Synonyms:

CGI-113; L11MT; MRP-L11

SwissProt:

Q9Y3B7

IHC positive control:

carcinoma of human thyroid tissue and adenocarcinoma of human colon tissue; adenocarcinoma of human ovary tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

21 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

500-2000