小鼠抗MRPL15單克隆抗體

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q.

Applications:

WB, IHC

Name of antibody:

MRPL15

Immunogen:

Fusion protein of human MRPL15

Full name:

mitochondrial ribosomal protein L15

Synonyms:

L15mt; RPML7; MRP-L7; HSPC145; MRP-L15

SwissProt:

Q9P015

IHC positive control:

Human breast cancer and Human ovarian cancer; Human endometrial cancer and Human lymph node tissue

IHC Recommend dilution:

200-500

WB Predicted band size:

33 KD

WB Positive control:

K562, 293T, MCF7 and Hela cell lysates

WB Recommended dilution:

200-1000